IHC for formalin fixed cells
I am trying to stain cells in matrigel 3 dimensional cultures for E_Cadherin and prostate specific membrane antigen. I fix my cultures ( Cells and matrigel) in formalin then embed them in OCT(Optimum cutting temperatur) making frozen sections from them. If I stain do I need to retrieve the antigen by heating in Citrate solution or not.
Re: IHC for formalin fixed cells
After fixing in formalin, you will might need antigen retrieval, but not necessarily.
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