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-   -   IHC for formalin fixed cells (http://www.molecularstation.com/forum/immunohistochemistry-forum/56229-ihc-formalin-fixed-cells.html)

salhasan2003 02-05-2009 10:29 PM

IHC for formalin fixed cells
 
I am trying to stain cells in matrigel 3 dimensional cultures for E_Cadherin and prostate specific membrane antigen. I fix my cultures ( Cells and matrigel) in formalin then embed them in OCT(Optimum cutting temperatur) making frozen sections from them. If I stain do I need to retrieve the antigen by heating in Citrate solution or not.

Thanks,

radam2k 04-23-2009 10:18 PM

Re: IHC for formalin fixed cells
 
After fixing in formalin, you will might need antigen retrieval, but not necessarily.


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