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Immunohistochemistry Forum Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


skin keratin background staining IHC

skin keratin background staining IHC - Immunohistochemistry Forum

skin keratin background staining IHC - Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


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Old 12-03-2008, 11:59 PM
Pipette Filler
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Exclamation skin keratin background staining IHC



Is there any method to avoid (to block) the unespecific staining of the skin keratin in immunohistochemistry?
I have problems with some samples of mouse skin (FFPE), Im interested in the uper layers of the epidermis so, with the unespecific staining of the keratin, I cant see if my staining in those cells is specific.
Im using Caspase 8 antibody from Abnova, citrate unmasking (Ph:6), peroxidase block, 1h of normal serum block,1:500 dilution of primary antibody and anti-rabbit Envision system from Dako. For the washes , TBS-T.
My positive control is cerebelum.
Thank you
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