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| Immunohistochemistry Forum Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections. |
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#1
12-03-2008, 10:59 PM
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 skin keratin background staining IHC Is there any method to avoid (to block) the unespecific staining of the skin keratin in immunohistochemistry? I have problems with some samples of mouse skin (FFPE), I´m interested in the uper layers of the epidermis so, with the unespecific staining of the keratin, I can´t see if my staining in those cells is specific. I´m using Caspase 8 antibody from Abnova, citrate unmasking (Ph:6), peroxidase block, 1h of normal serum block,1:500 dilution of primary antibody and anti-rabbit Envision system from Dako. For the washes , TBS-T. My positive control is cerebelum. Thank you  |
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| background , ihc , keratin , skin , staining |
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