Originally Posted by orestis
Hello, can we also reuse primary antibodies in immunohistochemistry IHC? I know that we can reuse primaries after westerns, but i would like to reuse the IHC antibodies to save money. Does anyone have experience in this?
Hi radam. Maybe you already solved the problem. We prepare dilutions for about 20-30 samples, with antibodies we use everyday, and they work for at least 1 month if you keep it in the fridge, and avoiding high temperatures, sunlight and a long time exposure to air oxidation. We also alicuotate dilutions for some special antibodies and keep them at -20 degrees.