Immunochemistry

sherly · #1 (**permalink**) 12-20-2007, 07:50 AM

Hai,
I am working on aromatase gene and from my RT PCR data i have seen that there is a very significant difference in the expression of the gene with age. can i use immunohistochemistry to study the protein expression and how can i quantify the expression? Since the gene expression is very different, can i expect similar significant difference in the staining pattern too? thanks in advance.
Regards,
Sherly.

danfive · #2 (**permalink**) 12-20-2007, 02:32 PM

To quantify you need reliable standards....Then you can use ELISA, WB, ISH.

sherly · #3 (**permalink**) 12-21-2007, 07:58 AM

Thankyou for the reply. but still can immunohistochemistry be used to study the increase in staining and thereby indicating an increase in the expression? or is it just to locate the expression site? i donot have sufficient quantity of the antibody to do WB. I am trying ISH, but isn't it also used as IHC to locate the expression site? if ISH can work, why is it that IHC cannot?

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Immunochemistry

Immunohistochemistry Forum

Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.