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Antibody for Melanin detection

Antibody for Melanin detection - Immunofluorescence Forum

Antibody for Melanin detection - Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


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Old 01-04-2013, 04:11 AM
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Default Antibody for Melanin detection



Hi,

I need to identify melanin particles (with high accuracy and resolution) in human skin using confocal microscopy . I tried reflectance mode but it hardly could be identified in this mode becuase I have a lot of bright dots in images 9I am using cadavar skin tissue not fresh).

So, I am thinking about using flourescence mode for detecting melanins. But I don't know if does the melanin flouresce (some papers say yes, some say no)? If it doesn't, is there any specific antibody for melanin that I could apply to detect melanin with high resolution and accuracy?

I am a researcher with engineering background, so any idea about anything in this area are welcome and much appreciated.

P.S. Does "particle" suits melanin?!

Thank you in advance for your help.
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Old 02-12-2013, 03:38 PM
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