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RSV detection by direct immunofluorescence

RSV detection by direct immunofluorescence - Immunofluorescence Forum

RSV detection by direct immunofluorescence - Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


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Old 11-28-2011, 06:25 PM
Pipette Filler
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Thumbs down RSV detection by direct immunofluorescence



I am doing research on RSV "Respiratory syncytial virus".
I'm using fluorescent antibodies to directly detect RSV antigens in nasopharyngeal secretions. I have several problems to deal wih, hope you can help me figure them out.

1. Can I use the standard glass slides, or I have to use the type that came with the wells and is acetone resistant? What is the standard protocol here?

2. For how long could I store the slides after fixing the sample with acetone? and at what temperature? I was told slides could be stored for several months at -40 C or below?

3. What is the thickness of the coverslip to use after staining step + adding mounting medium? And is it right I should use a varnish to protect the mounting medium from leaking out from beneath the coverslip?

Your help would really be appreciated
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