Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Histology Forum > Immunofluorescence Forum
Register Search Today's Posts Mark Forums Read

Immunofluorescence Forum Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


Lines in Images---what could cause this?

Lines in Images---what could cause this? - Immunofluorescence Forum

Lines in Images---what could cause this? - Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-22-2011, 06:22 PM
Pipette Filler
Points: 404, Level: 8 Points: 404, Level: 8 Points: 404, Level: 8
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2011
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Lines in Images---what could cause this?



Hi, I am cutting mouse brain sections to 10um on a cryostat (perfused in paraformaldehyde and frozen into a block of OCT), staining them with various antibodies, and visualising using a Zeiss 510 confocal microscope.

I have been finding that some of my images have black lines running across them; an example of this is shown in the image at srcf.ucam.org/~ns452/lines.JPG (address needs those three letters put before it... but I can't put them there due to spam filter).

If I move in the image, then the lines stay where they are, so I take that to mean that it is actually something present on the slide.... but it is not visible using bright-field mode.

Any suggestions on what this could be? Could it be caused by my cryostat technique?

Thanks
Reply With Quote
  #2  
Old 10-06-2011, 10:50 AM
Pipette Filler
Points: 617, Level: 12 Points: 617, Level: 12 Points: 617, Level: 12
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2010
Posts: 11
Thanks: 1
Thanked 3 Times in 3 Posts
Default Re: Lines in Images---what could cause this?

Sorry I can't open the image, but as the ZEISS 510 is a old confocal (we are using the same equipment as you do), I suppose that there is a problem with the scanning unit. I would try first to switch off the bidirectional scanning (if it is activated) and then decrease the scan speed. Maybe it is not on the slide...
Reply With Quote
The Following User Says Thank You to 5Ál-pipette_death_punch For This Useful Post:
admin (10-23-2011)
  #3  
Old 03-12-2013, 09:13 PM
haisond's Avatar
Pipette Filler
Points: 80, Level: 1 Points: 80, Level: 1 Points: 80, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2013
Location: los angeles, CA
Posts: 14
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Lines in Images---what could cause this?

I've experienced similar lines while making adjustments for the autofocus mode. The lines are generated from the laser burning or photobleaching while you are setting the autofocus parameters. This effect can be eliminated by reducing your laser power, and number of scans.
Reply With Quote
Reply

Tags
imageswhat , lines


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Where the universe is. T-minus108 Physics Forum 21 04-23-2008 08:30 PM
Drosophila Isofemale Lines Available Stacy Marie Mazzalupo Drosophila Forum 0 03-01-2007 03:38 AM
Breast cancer cell lines as models for breast tumors: a review scimedweb Cell Biology and Cell Culture 0 02-10-2004 10:21 AM
Seeing Holographically cinquirer Physics Forum 24 11-19-2003 12:09 PM
Images of Endocrine-active Changes Reimschuessel, Renate Zebrafish Forum 0 09-09-2003 05:54 PM


All times are GMT. The time now is 03:58 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13350 seconds with 16 queries