Hi, I am cutting mouse brain sections to 10um on a cryostat (perfused in paraformaldehyde and frozen into a block of OCT), staining them with various antibodies, and visualising using a Zeiss 510 confocal microscope.
I have been finding that some of my images have black lines running across them; an example of this is shown in the image at srcf.ucam.org/~ns452/lines.JPG (address needs those three letters put before it... but I can't put them there due to spam filter).
If I move in the image, then the lines stay where they are, so I take that to mean that it is actually something present on the slide.... but it is not visible using bright-field mode.
Any suggestions on what this could be? Could it be caused by my cryostat technique?