Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Histology Forum > Immunofluorescence Forum
Register Search Today's Posts Mark Forums Read

Immunofluorescence Forum Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


Flag Immunostainings on HEK 293 cells

Flag Immunostainings on HEK 293 cells - Immunofluorescence Forum

Flag Immunostainings on HEK 293 cells - Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-05-2010, 09:48 PM
Pipette Filler
Points: 141, Level: 2 Points: 141, Level: 2 Points: 141, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Flag Immunostainings on HEK 293 cells



Hello Everybody,

I am a new member to this forum. I have two main hurdles on my way to perform immunoflurescence experiments.
Firstly, the cell line which i have to use is HEK 293 cells- which seem to dettach very easily( despite of using collagen coated coverslips) during various steps of stainings especially when detergent are used.

Secondly, I need to use Flag antibody,M2(sigma) as primary antibody to stain a Flag tagged nuclear protein in my stainings which is further adding up to trouble with background staining or non-specific binding.

If anybody has any experience of Flag immunostainings for nuclear proteins and in HEK293 cells- please give your suggestions.

I wish...to get a good feedback on this query.
Reply With Quote
Reply

Tags
293 , cells , flag , hek , immunostainings


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-26-2011 11:40 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
How to isolate adult from circulation? peterish Stem Cell Forum 0 05-19-2008 10:02 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 06:47 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14585 seconds with 16 queries