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| Immunofluorescence Forum Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells. |
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#1
07-05-2010, 09:48 PM
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 Flag Immunostainings on HEK 293 cells Hello Everybody, I am a new member to this forum. I have two main hurdles on my way to perform immunoflurescence experiments. Firstly, the cell line which i have to use is HEK 293 cells- which seem to dettach very easily( despite of using collagen coated coverslips) during various steps of stainings especially when detergent are used. Secondly, I need to use Flag antibody,M2(sigma) as primary antibody to stain a Flag tagged nuclear protein in my stainings which is further adding up to trouble with background staining or non-specific binding. If anybody has any experience of Flag immunostainings for nuclear proteins and in HEK293 cells- please give your suggestions. I wish...to get a good feedback on this query.  |
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| 293 , cells , flag , hek , immunostainings |
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