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cy3 interaction with collagen gels

cy3 interaction with collagen gels - Immunofluorescence Forum

cy3 interaction with collagen gels - Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


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Old 04-23-2009, 03:36 PM
Pipette Filler
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Default cy3 interaction with collagen gels



I'm using anti-mouse Cy3 as a secondary antibody for staining cells encapsulated in a collagen gel. When we try to image the gels, there is a lot of background. We believe that Cy3 is interacting with the gel or that the dye doesn't have enough time to diffuse. Any suggestions on how to fix this? Thanks!
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