a question regarding fluorescent microscope.. or whatever
When I use a rhodamine conjugated antibody, should I see something trough a FITC filter as well???
Its very confusing to me, because, when I use FITC filter I see a signal just like i would of see it if i have used a FITC conjugated antibody,not in that intensity though, only regarding the position on nuclei. But I used rhodamine conjugated antibody!!
Is this normal? Should I see some green stains, or should I see only black in microscope?
Like this I could never use incubation with 2 antibodies at the same time
thank you very much for some answer