|Register||Search||Today's Posts||Mark Forums Read|
|Immunofluorescence Forum Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.|
| ||LinkBack||Thread Tools||Display Modes|
a question regarding fluorescent microscope.. or whatever
When I use a rhodamine conjugated antibody, should I see something trough a FITC filter as well???
Its very confusing to me, because, when I use FITC filter I see a signal just like i would of see it if i have used a FITC conjugated antibody,not in that intensity though, only regarding the position on nuclei. But I used rhodamine conjugated antibody!!
Is this normal? Should I see some green stains, or should I see only black in microscope?
Like this I could never use incubation with 2 antibodies at the same time
thank you very much for some answer
|fluorescent , microscope|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Nikon Microscope - Eclipse 200 Best Clinical Scope?||Microscope Co||Lab Equipment Discussion and Reviews||1||04-18-2012 03:29 AM|
|Choosing A Microscope : Compound or Stereo microscope?||nationalmicroscope||Histology Forum||0||01-19-2010 07:11 AM|
|Scanning Microscope, Confocal Scanning Microscope and Transmission Electron...||Mustafa M||Confocal - Microscopy Imaging Techniques||1||08-06-2009 10:08 AM|
|fluorescent microscope?||Nazaire J||Confocal - Microscopy Imaging Techniques||2||04-01-2007 10:59 PM|
|Fluorescent Microscope?||Alan Blake||Zebrafish Forum||0||11-11-2005 03:53 AM|