Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Histology Forum > Immunofluorescence Forum
Register Search Today's Posts Mark Forums Read

Immunofluorescence Forum Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


Fluorescent microscope

Fluorescent microscope - Immunofluorescence Forum

Fluorescent microscope - Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-31-2009, 08:57 AM
Pipette Filler
Points: 900, Level: 16 Points: 900, Level: 16 Points: 900, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2009
Posts: 7
Thanks: 0
Thanked 1 Time in 1 Post
Default Fluorescent microscope



Hi,

a question regarding fluorescent microscope.. or whatever
When I use a rhodamine conjugated antibody, should I see something trough a FITC filter as well???
Its very confusing to me, because, when I use FITC filter I see a signal just like i would of see it if i have used a FITC conjugated antibody,not in that intensity though, only regarding the position on nuclei. But I used rhodamine conjugated antibody!!
Is this normal? Should I see some green stains, or should I see only black in microscope?
Like this I could never use incubation with 2 antibodies at the same time

thank you very much for some answer
Reply With Quote
Reply

Tags
fluorescent , microscope


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Nikon Microscope - Eclipse 200 Best Clinical Scope? Microscope Co Lab Equipment Discussion and Reviews 1 04-18-2012 03:29 AM
Choosing A Microscope : Compound or Stereo microscope? nationalmicroscope Histology Forum 0 01-19-2010 08:11 AM
Scanning Microscope, Confocal Scanning Microscope and Transmission Electron... Mustafa M Confocal - Microscopy Imaging Techniques 1 08-06-2009 10:08 AM
fluorescent microscope? Nazaire J Confocal - Microscopy Imaging Techniques 2 04-01-2007 10:59 PM
Fluorescent Microscope? Alan Blake Zebrafish Forum 0 11-11-2005 04:53 AM


All times are GMT. The time now is 03:51 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11311 seconds with 16 queries