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#1
03-31-2009, 08:57 AM
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 Fluorescent microscope Hi, a question regarding fluorescent microscope.. or whatever  When I use a rhodamine conjugated antibody, should I see something trough a FITC filter as well??? Its very confusing to me, because, when I use FITC filter I see a signal just like i would of see it if i have used a FITC conjugated antibody,not in that intensity though, only regarding the position on nuclei. But I used rhodamine conjugated antibody!! Is this normal? Should I see some green stains, or should I see only black in microscope? Like this I could never use incubation with 2 antibodies at the same time thank you very much for some answer  |
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