Methanol Fixation of SH-SY5Y cells for Immunofluorescence
 

Hi,
Im using methanol to fix SH-SY5Y cells, and stain nuclear with DAPI later. I am using the following conditions: ice-cold methanol fix for 5 min

However I found DAPI stain sometimes goes out of the nuclear and looks fluffy. I have no idea what is wrong could someone please help me,
thanks.

Re: Methanol Fixation of SH-SY5Y cells for Immunofluorescence
 

Hi there, we often use formaldehyde or PFA for fixing cells (SHSY5Y). Why don't you try one of these ?
cheers

Re: Methanol Fixation of SH-SY5Y cells for Immunofluorescence
 

you can try Carnoy's fixative(1 part glacial acetic acid to 3 parts methanol).