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Immunofluorescence Forum Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.


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Immunofluorescence Nuclear Staining

Immunofluorescence Nuclear Staining - Immunofluorescence Forum

Immunofluorescence Nuclear Staining - Immunofluorescence Forum. Talk, discuss and post questions on immunofluorescence techniques including paraffin, frozen sections or cultured cells.



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Old 05-21-2008, 05:17 AM
Pipette Filler
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Default Immunofluorescence Nuclear Staining

Hiya
In tissue sections i have been trying to stain nuclei with a blue fluorophore with slides treated with 2N HCl for BrdU for an indirect immunofluorescence detection method.
Using PI, SYTOX , DAPI, and Hoeschst 33342 stains has not been successful.

Does anybody know of a good stain for denatured DNA in nuclei?
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