Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Histology Forum
Register Search Today's Posts Mark Forums Read

Histology Forum Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.


Freezing HCAEC's and IF

Freezing HCAEC's and IF - Histology Forum

Freezing HCAEC's and IF - Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-04-2012, 09:37 AM
Pipette Filler
Points: 14, Level: 1 Points: 14, Level: 1 Points: 14, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Freezing HCAEC's and IF



Hi, I'm new here, and I have an interesting problem that I'm hoping someone can shed light on! Basically, I'm growing human coronary artery cells for IF (using labtek II chamber slides). I'm using gp340 as a primary and alexa 594 as secondary. After trying various fixatives, acetone is the best option for me(as pfa requires pre treatment and is too harsh on the cells). The other day I froze a slide in the -80, and did my IF the following week. The result was really positive in the frozen slide, but not in the fresh fixed. Does anyone know anything about the possibility of freezing making cells more permeable? Thank you!
Reply With Quote
Reply

Tags
freezing , hcaec


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 09:29 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10625 seconds with 16 queries