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"cloudy" microscopic appearance of H&E sections

"cloudy" microscopic appearance of H&E sections - Histology Forum

"cloudy" microscopic appearance of H&E sections - Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.


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Old 08-01-2011, 12:45 AM
Pipette Filler
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Question "cloudy" microscopic appearance of H&E sections




I would be grateful if you can shed light on a phenomenon that is grieving our Pathology Director - The appearance of some of our H&E sections; eg. mucosal bx's - have a "cloudy" or "veiled" appearance under the microscope. The staining protocol has been modified and much improved so less attention is drawn to this but I have to concede that this appearance does show up intermittently on daily surgicals. Processing temperatures of dehydrating alcohols and xylenes on the VIP Processor are now 35 degrees (were 40) and fixation appears not to be an issue. De-paraffinisation times have also been increased on our Leica Stainer.

1) Fixation?
2) Processing?
3) Staining?
4) Dirty coverslips?
5) " Objectives?
6) Mountant?
7) insufficient time/change of dehydrating solutions on Stainer?
8) any Suggestions?

Thanks for helping.
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