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-   -   Tissue Sectioning for Mass Spec - Please Help (http://www.molecularstation.com/forum/histology-forum/77562-tissue-sectioning-mass-spec-please-help.html)

philliesfan 04-05-2011 07:48 PM

Tissue Sectioning for Mass Spec - Please Help
 
Hey guys,

I was just wondering if anyone out there has experience cryosectioning for mass spec analysis? I have several mouse tissues I want to embed: kidney, brain, lung, heart, and spleen. I have read that TissueTek OCT compound because it is a polymeric compound - it can suppress ion signal during mass spec. analysis.

Has anyone have any experience in the following:
- Ice Slush - I am not quite sure what exactly this is but I am assuming you either freeze the tissue in a block of distilled water or use water as "glue" to adhere tissue to the chuck?
- 10% Gelatin - it seems to have the same consistency as the OCT compound when frozen.
- 2% aqeuous carboxymethyl cellulose embedding media

I can try my luck sectioning without embedding media; however, I need sections that are >40 microns. This could pose a problem sectioning without media. It's hard enough to section brain with OCT media, I can't imagine how difficult this would be WITHOUT any media at all.

I am also open to other non-polymeric embedding media.

All suggestions are greatly appreciated :)

With regards,

Philliesfan


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