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Question about simple cytoplasmic staining.
Doing some studying from Carson and Hladik's Histotechnology 3rd edition. So if anyone is familiar with this text, I'd would greatly appreciate some help. I believe 2nd edition is very similar as well.
On page 107, second column, Cytoplasmic Staining....there is FIGURE 6.1 which depicts the structural formula for a simple amino acid and the effect of its isoelectric point (pH 6). I'm confused why the amino acid is listed as "basic (+)" with a lower pH and "acidic (-)" at a higher pH. A simple definition of acid (Lewis or Bronstead-Lowry?) says that an acid is any substance that can donate a H+ ion. This seems to be the case for the amino acid at the lower pH, since the amino and carboxyl groups are both +, meaning it would very readily react with a base, which is a substance that accepts H+. So why the reverse of the classifications in the figure?
The next page (108, final paragraph) even says:
So again, in FIGURE 6.1 on 107, why is the amino acid listed as "basic (+)" at the lower pH and "acidic (-)" at the higher pH?
Thanks for any help. Sorry I don't have a picture to post.
Last edited by GrimmHatter; 03-19-2011 at 02:58 AM.
|cytoplasmic , question , simple , staining|
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