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brittle bones

brittle bones - Histology Forum

brittle bones - Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.


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Old 12-16-2010, 01:28 PM
Pipette Filler
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Default brittle bones



I'm experiencing a problem with bones.
When I cut the sections, the bone is very brittle, and the morphology isn't conserved. Is this due to the decalcification (not enough time, too long?, using EDTA pH 7.3), or the parafin perhaps? Problem during embedding in parafin? The de/hydration process?
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