H& E staining

[Svk25]

Hi,
Brain sections (20-40 microns) are falling off from Fisher charged slides at post-fixing step with 10% formalin itself or/and with increasing % of water at rehydration during H& E staining. They are being stained 2-3 hours after cutting the brain.
Does anyone have ideas of what could be going wrong and how it could be prevented?
Thanks!

[shmily10]

i have a section tissue by cutting frozen. Skin's mouse is fixed in tissue freezing medium and cutted by leica -microsystem. Then, the sestion is stainned with H&E. I pratice with protocol adler lab protocol (H&E staining for frozen tissue section).
The image is very bad. I think have a problem. Can you help me. Thanks!
image:mediafire.com/?z2zt5gtd3md

[mrsibar]

@svk25 Hi i do regular histology sections for rodent tissues and sections are washed off from slides usually if u are using water at more force adjust the force of running water or just dip in water to wash

[mrsibar]

@shmily 10 Hi frozen sections have this problem if they are stored for a long time
Cryosections are only for temporary use for better images always go for formalin fixed paraffin sections