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Adipose tissue problem

Adipose tissue problem - Histology Forum

Adipose tissue problem - Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.


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Old 11-04-2009, 06:54 AM
Pipette Filler
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Default Adipose tissue problem



Hi friends,

I started to isolate proteins from human adipose tissue. But, I am facing a severe problem with the blood cells/blood vessels. So, I eventually happened to get a lysate which is red in colour. As I am using BCA protein assay kit for the assay of proteins, I am getting false results due to the color interference. I am clueless how to get rid of this color. I have very little time left to perform this experiment and my supervisor is awaiting my results. Pleaaaaaase send me any replies. I really appreciate any suggestions to my problem.
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