Histology ForumHistology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.
Cryosections - Histology Forum
Cryosections - Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.
I am grateful to anyone who helps me answer the following queries:
a. What is the chemical composition of O.C.T or tissue embedding medium?
b. What is a good way to collect cryosections onto slides? what are they coated with to ensure the sections adhere to the slide? Are such slides commercially available?
Hi sarita we dont require anything to make sectons adhere to slides .I use normal glass slides and make 8um sections after sectioning i fix in 70percent alcohol they wont usually come off the slide .What protocol are u following for storing tissues for cryosections?
The Following User Says Thank You to mrsibar For This Useful Post:
I have used TissueTek OCT compound (Sakura 4583) and it contains 10% polyvinyl alcohol, 4% polyethylene glycol, and the rest is nonreactive ingredients according to the bottle. I used positively charged coated slides (Superfrost). Other options could be coating your own slides with poly-L-Lysine. I just touch the slide to the tissue gently to adhere to the slide, just make sure you keep your slide at room temperature. The tissue won't stick if the slide is frozen.
The best reference for histology and cryosectioning techniques I recommend: Carson, Freida L, Histotechnology a Self-Instructional Text. 3rd Edition. (ISBN:978-0-89189-581-7). They give excellent protocols, discuss the theory, and they even have an excellent troubleshooting section with pictures at the end of each chapter.
I hope this helps! Good Luck!
Laboratory Technician at Protea Biosciences, Inc. [Only registered users see links. ]
Last edited by proteaPam; 03-24-2011 at 02:45 PM.
The Following User Says Thank You to proteaPam For This Useful Post: