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Counterstains Alternatives to Hematoxylin

Counterstains Alternatives to Hematoxylin - Histology Forum

Counterstains Alternatives to Hematoxylin - Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.


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Old 01-28-2009, 04:44 AM
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Question Counterstains Alternatives to Hematoxylin



Hi, Im looking for a counter-stain for some sections that I have stained with NBT/BCIP (purple). I don't want to use hematoxylin, since this is too close to the NBT/BCIP stain. Does anyone have any ideas?

I've been looking at trying Nuclear Fast red (also known as Kernechtrot stain), but the few photos I can find on the internet do not show very good staining. Has anyone tried this, and if so, did it work well for you? If not, are there any alternatives? I do not want to sue a fluorescent counterstian (eg. DAPI etc).
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Old 03-11-2013, 09:37 PM
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Default Re: Counterstains Alternatives to Hematoxylin

try Methyl Green (stain cells green) reagent source: Vector Labs, protocol: IHC World.
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