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Stain Dead Cells

Stain Dead Cells - Histology Forum

Stain Dead Cells - Histology Forum. Talk, discuss and post questions on general histology methods including tissue processing, embedding tissues, sectioning, special staining, enzyme staining and silver staining of samples.


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  #1  
Old 07-11-2008, 10:49 PM
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Default Stain Dead Cells



Hi everyone!
I'm interested in staining dead cells in their cell culture before fixing them. I have the LiveDead cell kit from Invitrogen which stains dead cells but Im not sure if the stained cells can be fixed and if they can how and also keep the red fluorescence after the fixation step?

ANy tips anyone can give me or other methods I should try?

Thanks
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Old 03-11-2013, 09:33 PM
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Default Re: Stain Dead Cells

Propidium Iodide is a good fluorescent dye for staining dead cells, but you will need a rhodamine filter to view or quantify the dead cells. Trypan Blue is another alternative
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Old 09-05-2013, 08:07 AM
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Default Re: Stain Dead Cells

Trypan blue : are not a good alternative in how much can not distinguish necrotic vs. terminally apoptotic cells.
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