Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Histology Forum (http://www.molecularstation.com/forum/histology-forum/)
-   -   Stain Dead Cells (http://www.molecularstation.com/forum/histology-forum/3570-stain-dead-cells.html)

FigEater 07-11-2008 10:49 PM

Stain Dead Cells
 
Hi everyone!
I'm interested in staining dead cells in their cell culture before fixing them. I have the LiveDead cell kit from Invitrogen which stains dead cells but Im not sure if the stained cells can be fixed and if they can how and also keep the red fluorescence after the fixation step?

ANy tips anyone can give me or other methods I should try?

Thanks:thumbs:

haisond 03-11-2013 09:33 PM

Re: Stain Dead Cells
 
Propidium Iodide is a good fluorescent dye for staining dead cells, but you will need a rhodamine filter to view or quantify the dead cells. Trypan Blue is another alternative

Zagami 09-05-2013 08:07 AM

Re: Stain Dead Cells
 
Trypan blue : are not a good alternative in how much can not distinguish necrotic vs. terminally apoptotic cells.


All times are GMT. The time now is 04:10 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09071 seconds with 11 queries