Hi, my lab has absolutely no experience performing genome-wide yeast screens. I'm thinking I would like to do this kind of analysis to screen for genes important in resistance to a specific drug as a part of my dissertation. I am in the planning process now.
Can someone tell me a little about how labor intensive this is? Specifically, I am interested in performing the pooling studies with barcoded genes, as I understand that this type of screen is a little less labor intensive. How many months would I spend working at the bench to do this as a novice?
Also, how expensive do these screens tend to be? We also are a little poor right now. Is it pricey? If anyone has suggestions on other methods that would allow me to get these types of data for a cheaper price I'd be interested in hearing that also.