Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > General Science Questions and Layperson Board
Register Search Today's Posts Mark Forums Read

General Science Questions and Layperson Board General Science Questions and Layperson Board


Effect of pH on staining

Effect of pH on staining - General Science Questions and Layperson Board

Effect of pH on staining - General Science Questions and Layperson Board


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-14-2008, 08:25 PM
Pipette Filler
Points: 204, Level: 4 Points: 204, Level: 4 Points: 204, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2008
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Effect of pH on staining



Does anyone know the effect of staining a sample with a acidic dye such as eosin in acid and alkaline conditions? Thanks
Reply With Quote
  #2  
Old 10-21-2008, 09:50 AM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Default Re: Effect of pH on staining

Hello Dan,
welcome to the forums. What sample do you wish to stain: protein or nucleic acid or tissue?
more information on Eosin staining:
Eosin is a fluorescent red dye resulting from the action of bromine on fluorescein. It can be used to stain cytoplasm, collagen and muscle fibers for examination under the microscope. Structures that stain readily with eosin are termed eosinophilic.
Reply With Quote
  #3  
Old 12-03-2008, 03:42 AM
butters's Avatar
M.D/Ph.D
Points: 4,938, Level: 47 Points: 4,938, Level: 47 Points: 4,938, Level: 47
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2008
Location: Malaysia
Posts: 315
Thanks: 25
Thanked 68 Times in 63 Posts
Default Re: Effect of pH on staining

As I remember acidic dye will bind to alkaline region in sample; neutralization. staining in different pH condition will change the intensity of the stain. I could be wrong but this is so far I know
Reply With Quote
Reply

Tags
effect , staining


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
What is Gravity? sdr@sdrodrian.com Physics Forum 1 01-27-2008 02:53 PM
Photoelectric effect is not quantized h_v_ansari@yahoo.com Physics Forum 1 10-26-2006 01:23 PM
Logical justification of the Hall effect h_v_ansari@yahoo.com Physics Forum 1 10-19-2006 07:23 PM
Meissner Effect preclude a "self-created current" ?? Archimedes Plutonium Chemistry Forum 6 10-11-2003 12:14 PM


All times are GMT. The time now is 10:04 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14550 seconds with 16 queries