Effect of pH on staining

[Dan167]

Does anyone know the effect of staining a sample with a acidic dye such as eosin in acid and alkaline conditions? Thanks

[admin]

Hello Dan,
welcome to the forums. What sample do you wish to stain: protein or nucleic acid or tissue?
more information on Eosin staining:
Eosin is a fluorescent red dye resulting from the action of bromine on fluorescein. It can be used to stain cytoplasm, collagen and muscle fibers for examination under the microscope. Structures that stain readily with eosin are termed eosinophilic.

[butters]

As I remember acidic dye will bind to alkaline region in sample; neutralization. staining in different pH condition will change the intensity of the stain. I could be wrong but this is so far I know