I am desperate for some advice and help (please) concerning concentrations as I have got myself very confused. I have been supplied with some chemical at 50% concentrate. The company have already done tests stating that they think it works at a 1:250 dilution from their 100% concentrate. So surely this is a 0.4% solution?
Part of my protocol states that I need to use the working concentration of this chemical to do the necessary tests, which according to the company is as above, 1:250. However I was told by a colleague that all I need to do is take my 50% concentrate and do a 1:250 dilution. This surely will not give me the same concentration as the company has used in their tests as they have a 100% concentrate to start with and I have 50%??
As I am writing this I appreciate it is a really stupid question however my supervisor has told me that the concentration does not matter and this has really got me confused, but I think this does matter. How do I get the same concentration the company worked with from my 50% dilution? If I diluted my 50% concentrate to 1:250 what is the % chemical?
Thanks in advance for any help