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Concentration - General Science Questions and Layperson Board

Concentration - General Science Questions and Layperson Board

#1
06-04-2014, 12:26 AM
Pipette Filler

Join Date: Jun 2014
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Concentration

Hi
I am desperate for some advice and help (please) concerning concentrations as I have got myself very confused. I have been supplied with some chemical at 50% concentrate. The company have already done tests stating that they think it works at a 1:250 dilution from their 100% concentrate. So surely this is a 0.4% solution?

Part of my protocol states that I need to use the working concentration of this chemical to do the necessary tests, which according to the company is as above, 1:250. However I was told by a colleague that all I need to do is take my 50% concentrate and do a 1:250 dilution. This surely will not give me the same concentration as the company has used in their tests as they have a 100% concentrate to start with and I have 50%??

As I am writing this I appreciate it is a really stupid question however my supervisor has told me that the concentration does not matter and this has really got me confused, but I think this does matter. How do I get the same concentration the company worked with from my 50% dilution? If I diluted my 50% concentrate to 1:250 what is the % chemical?

Thanks in advance for any help
#2
06-04-2014, 06:54 PM
M.D/Ph.D

Join Date: Jul 2010
Location: Mexico
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Re: Concentration

Easy, if the co. uses 1:250 from a 100% then you should cut in half the dilution factor:

100% diluted 250 = 0.4% = 1X solution (WORK SOLUTION)

50% diluted 250 = 0.2% = 0.5X solution (diluted 1:2 work solution) = WRONG Solution

Cut in half the dilution factor:
 The Following User Says Thank You to luisillo For This Useful Post: microbunny (06-04-2014)
#3
06-04-2014, 06:57 PM
M.D/Ph.D

Join Date: Jul 2010
Location: Mexico
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Re: Concentration

50% diluted 125 = 0.4% = 1X solution (WORK SOLUTION)

In conclusion, you have to use a 1:125 dilution.

Good luck
 The Following User Says Thank You to luisillo For This Useful Post: microbunny (06-04-2014)
#4
06-04-2014, 11:32 PM
Pipette Filler

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Re: Concentration

Hi luisillo

You are a genius. Thank you so much for explaining it so clearly.
#5
06-06-2014, 05:05 PM
M.D/Ph.D

Join Date: Jul 2010
Location: Mexico
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Re: Concentration

Your welcome. Thanks for the compliment and sorry for answering in two posts, I accidentally hit the "post reply" button before ending writing.
#6
06-16-2014, 02:04 AM
M.D/Ph.D

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Re: Concentration

I am late but I would just like to say, usually for concentration I refer to the epic formula M1V1=M2V2

Since you know your M2 is 0.4% and M1, 50% (product you received).

Remember M1V1 = M2V2
 The Following User Says Thank You to butters For This Useful Post: microbunny (06-18-2014)
#7
06-18-2014, 06:44 PM
Pipette Filler

Join Date: Jun 2014
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Re: Concentration

Hi butters

A truly epic formula indeed! Thank you so much. I have embedded in my brain for future concentration shenanigans.

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