Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > General Science Questions and Layperson Board
Register Search Today's Posts Mark Forums Read

General Science Questions and Layperson Board General Science Questions and Layperson Board


Microarray normalisation for calculating correlation coefficients

Microarray normalisation for calculating correlation coefficients - General Science Questions and Layperson Board

Microarray normalisation for calculating correlation coefficients - General Science Questions and Layperson Board


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-27-2014, 10:14 AM
Pipette Filler
Points: 24, Level: 1 Points: 24, Level: 1 Points: 24, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2014
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Microarray normalisation for calculating correlation coefficients



Hi,
I am trying to do microarray analysis on a time-series (5 time points) gene expression (GE) data of 3000 genes. i want to calculate all Vs all (3000 Vs 3000 genes') Pearson correlation coefficient values. I searched a lot in literature and found that some studies normalize the data taking ratios of GE intensity values of individual genes and then Log transform these values, while other studies subtract the control GE intensity value of a gene from the test value. Please guide me on what is the appropriate way of normalisation that would suit my need.

Thanking you in anticipation,
Vipul N.
Reply With Quote
Reply


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Analysis of correlation between microarray and qPCR mschmidt Microarrays Forum 0 01-20-2011 09:30 AM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
qPCR NEWS April 2009 - focus on microRNA normalisation Editor www.Gene-Quantification.info Protocols and Methods Forum 0 04-29-2009 02:18 PM
slide assay with vista green. It's possible? gberna Protocols and Methods Forum 18 04-21-2006 02:39 PM


All times are GMT. The time now is 01:49 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12715 seconds with 16 queries