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Inconsistent Growth in E. coli Transformants

Inconsistent Growth in E. coli Transformants - General Science Questions and Layperson Board

Inconsistent Growth in E. coli Transformants - General Science Questions and Layperson Board


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Old 09-04-2013, 03:09 AM
Pipette Filler
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Default Inconsistent Growth in E. coli Transformants



I am studying some wild-type and mutants of E. coli. After electroporating with an empty ampicillin plasmid, a very obvious inconsistent colony size is observed. I have gone through all of the stocks, DNA preps, AMP plates, etc. and can find no contamination anywhere. For some reason, the inconsistent colony size is only seen in transformants, and not in the stocks of wild-type or mutant. They look nothing like satellite colonies.

What do you think could be the reason for this phenomenon?
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