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benzonase to get rid of nucleic acids

benzonase to get rid of nucleic acids - General Science Questions and Layperson Board

benzonase to get rid of nucleic acids - General Science Questions and Layperson Board


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Old 04-26-2013, 08:54 AM
Pipette Filler
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Default benzonase to get rid of nucleic acids



Hi everybody!
I wanted to ask whether somebody have used benzonase to shear DNA and to solubilize a protein from a chromatin pellet.
I needed a "protocol" just to know incubation times, how many units to use (Istill have to order it, maybe there are alternatives).
After shearing DNA I just want to load my protein on SDS-PAGE, my final goal is to test whether an interaction requires DNA to take place, so I want to get rid of DNA.
Thanks a lot for any answer!
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benzonase , chromatin pellet , s. cerevisiae


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