DNA digestion, ligation
I am facing problem at transformation step. After ligation I am not able to get the colonies. I change buffer, media and enzyme. Start getting colonies but those are not positive colonies. I have done digestion with Hind111 and XHO1. at 37 for 2hr then purified by qiagen gel extraction and ligate overnight at 4 oC.
My all grown positive colonies are showing smearing. What could be the reason. How I can troubleshoot this problem.
|All times are GMT. The time now is 11:35 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved