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Hematology auto slide-stainer

Hematology auto slide-stainer - General Science Questions and Layperson Board

Hematology auto slide-stainer - General Science Questions and Layperson Board


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  #1  
Old 07-19-2012, 01:20 PM
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Default Hematology auto slide-stainer



Hello everyone,

I work for a small laboratory that is looking to replace our old Hema-tek automatic slide stainer. We have been doing some research and have our list narrowed down to 3 makes that we are interested in, but would like some feedback on if anyone has any experience with any of the models.
1) IUL Poly-Stainer
2) Wescor Aerospray
3) Bayer/Siemens Hema-Tek 2000

I would appreciate any guidance you can give me!

Michelle
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Old 03-12-2013, 09:55 AM
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Default Re: Hematology auto slide-stainer

Dear Michelle
If in the days when posted you have purchased a "Wescor Aerospray", due to the fact that working in a small laboratory where the costs have to be contained I send you specific procedure to home realize reagents for put on board of this instrument.
Romanowsky stained
Thiazin Stain : 35 % dH2O, 65 % methanol, 5.44 g/L Imidazole, 9.28 g/L Maleic Acid, 4.25 g/L KOH, 2.29 g/L Azure B, 1.15 g/L Methylene blue, 10 g/L Triton X-100 and adjust pH to 7.0
Eosin Stain : 35% dH2O, 65 % methanol, 1.51 g/L Eosin Y and adjust pH to 7.0
Rinse Buffer : 0.408 g/L Imidazole, 0.696 g/L Maleic Acid, 0.458 g/L KOH, 0.05 g/L Triton X-100 and adjust pH to 6.8
Let me know
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Old 03-14-2013, 09:39 AM
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Default Re: Hematology auto slide-stainer

Dear Michelle
A modified Wright's-Giemsa stain on Hema-Tek I slide stainer below is described
Reagents and position site on the instrument :
First pump station – Fixative : anhydrous methanol
Second pump station - Stain A : 0.25% methylene blue, 0.25% Azure B and 0.02% trialkyl benzyl ammonium chloride salt as myristalkonium chloride (dimethylmyristylbenzylammonium chloride) or benzyldimethyltetradecylammonium chloride (Fluka cod. 13401) or Benzalkonium chloride (Fluka cod. 12063) as preservative, in 100 ml dH2O.
Third pump station - Stain B : 0.073 % eosin Y, 0.4 % phosphate buffer at pH 6.875 0.125 (weight out accurately 0.2 g of KH2PO4 and 0.2 g of Na2HPO4 and dissolve in 50 ml dH2O), 12.5 % acetone and 6.25% dimethylformamide (DMF) and fill to 100 ml with dH2O.
Fourth pump station - Rinse reagent : 0.087% phosphate buffer at pH 6.8 (weight out accurately 0.436 g of KH2PO4 and 0.434 g of Na2HPO4 and dissolve in 900 ml dH2O), 0.01 % of trialkyl benzyl ammonium chloride salt, see above, as preservative and 0.1 % of Pluronic types F68 (Sigma cod. P1300) or F86LF that non-ionic surfactant polyoxyethylene-polyoxypropylene block copolymer they are, and fill to 1000 ml with dH2O.
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