Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > General Science Questions and Layperson Board
Register Search Today's Posts Mark Forums Read

General Science Questions and Layperson Board General Science Questions and Layperson Board


smear in pcr

smear in pcr - General Science Questions and Layperson Board

smear in pcr - General Science Questions and Layperson Board


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-08-2012, 05:26 AM
Pipette Filler
Points: 452, Level: 9 Points: 452, Level: 9 Points: 452, Level: 9
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2011
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default smear in pcr



I am getting severe problem of smear after PCR even in negative control when i only have template DNA of 100bp long. smear start from the well and it is so intense as hundreds of microgram DNA is digested. can anyone help me to rule out the reason. thanks
Reply With Quote
  #2  
Old 05-24-2012, 07:37 AM
Banned
Points: 308, Level: 6 Points: 308, Level: 6 Points: 308, Level: 6
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2012
Posts: 21
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: smear in pcr

when i only have template DNA of 100bp long. smear start from the well and it is so intense as hundreds of microgram DNA is digested. can anyone help me to rule out the reason.
Reply With Quote
Reply

Tags
pcr , smear


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
pcr smear bhaktamehul PCR - Polymerase Chain Reaction Forum 27 01-15-2013 08:57 AM
PCR Smear qpwoei4756 PCR - Polymerase Chain Reaction Forum 2 01-04-2012 08:27 PM
smear in pcr product, but of same size as that of positive product uru PCR - Polymerase Chain Reaction Forum 1 06-20-2011 10:58 PM
A mystery!!! Plasmids smear when digested with site-specific enzymes doug1c Molecular Cloning Forum 2 10-29-2010 01:11 AM
AFLP smear Yuma248 Protocols and Methods Forum 0 08-13-2009 10:12 AM


All times are GMT. The time now is 11:26 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11338 seconds with 16 queries