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260/280 ratio very high in RNA extraction

260/280 ratio very high in RNA extraction - General Science Questions and Layperson Board

260/280 ratio very high in RNA extraction - General Science Questions and Layperson Board


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Old 03-09-2012, 12:39 PM
Pipette Filler
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Default 260/280 ratio very high in RNA extraction



Hi,I extract RNA with Qiagen kit. I measure the conc. in nanodrop and then see the quality in Bioanalyzer. I get very good quality of RNA, totally intact rRNA. One thing I don't understand is that the ratio of 260/280 I get in nanodrop is always very high such as 2.3-2.5 and the other is even higher. I don't know how that happens. what is the significance of this high ratio?

thank you very much for the answers
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extraction , extraction or isolation , high , molecular biology , protocols , ratio , rna


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