260/280 ratio very high in RNA extraction
Hi,I extract RNA with Qiagen kit. I measure the conc. in nanodrop and then see the quality in Bioanalyzer. I get very good quality of RNA, totally intact rRNA. One thing I don't understand is that the ratio of 260/280 I get in nanodrop is always very high such as 2.3-2.5 and the other is even higher. I don't know how that happens. what is the significance of this high ratio?
thank you very much for the answers
|All times are GMT. The time now is 05:26 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved