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melanogaster 03-09-2012 12:39 PM

260/280 ratio very high in RNA extraction
 
Hi,I extract RNA with Qiagen kit. I measure the conc. in nanodrop and then see the quality in Bioanalyzer. I get very good quality of RNA, totally intact rRNA. One thing I don't understand is that the ratio of 260/280 I get in nanodrop is always very high such as 2.3-2.5 and the other is even higher. I don't know how that happens. what is the significance of this high ratio?

thank you very much for the answers


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