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Leptin receptor prep

Leptin receptor prep - General Science Questions and Layperson Board

Leptin receptor prep - General Science Questions and Layperson Board


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Old 03-06-2012, 11:34 AM
Pipette Filler
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Default Leptin receptor prep



Hi everyone!

I'm pretty much a beginner scientist, but I'll try to define my problem as clear as I can:
I'm working with rat brain (hypothalamus), and I've been trying to isolate the leptin receptor, which is a plasma membrane protein, so i could analyse it by western blot. Most times, the only bands I could detect were nonspecific (I could see specific bands a few times, but they were lighter than the nonspecific ones). It might be the quality of the antibody, but I think that it could also be the result of my not knowing the right way to isolate a membrane receptor from the brain tissue-using different preps gave me different quality of the specific bands. Any advice, anyone?

Thanks














, and have been trying to isolate and prepare a membrane protein
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