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Giana 01-23-2012 12:03 AM

Loop Mediated amplification question
 
Hi, I'm Giana Gomes, I'm new in this group. I work as a researcher worker for the Aquaculture Department from James Cook University-Australia.

I'm starting a project with the Lamp technique. I need to differentiate 2 species of fish using this technique. My question is, how many (minimum) base pairs do I need in the 4 (F3/B3 FIB/BIP) sets of primers to differentiate the 2 species? Is it enough to have just 1 or 2 different base pairs in just 1 or 2 of the 4 sets of primers to give me the specificity for each specie?

Thank you.

diagmole 03-07-2014 04:54 PM

Re: Loop Mediated amplification question
 
Quote:

Originally Posted by Giana (Post 438220)
Hi, I'm Giana Gomes, I'm new in this group. I work as a researcher worker for the Aquaculture Department from James Cook University-Australia.

I'm starting a project with the Lamp technique. I need to differentiate 2 species of fish using this technique. My question is, how many (minimum) base pairs do I need in the 4 (F3/B3 FIB/BIP) sets of primers to differentiate the 2 species? Is it enough to have just 1 or 2 different base pairs in just 1 or 2 of the 4 sets of primers to give me the specificity for each specie?

Thank you.

you will need a minimum of 4 primers


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