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Primer concentrations

Primer concentrations - General Science Questions and Layperson Board

Primer concentrations - General Science Questions and Layperson Board


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Old 01-18-2012, 11:55 AM
Pipette Filler
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Default Primer concentrations



Hi all,
I am new into this issue of designing primers and working with different concentrations of primers. I am not able to between micromolar, picomolar concentrations etc.

I ordered primers and they have come in lyophilised forms, with the following:
synthesis scale 40nmol
purification Desalted
No of bases 21

From literature, exact volumes for a reaction of 25ul is not given, although concentrations are given i.e. 1.6uM. and in another literature this is given as 50pmol.
How do I proceed from here and reach a desired volume for my reaction mix? Thanks in advance
edok
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