Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Gene Therapy
Register Search Today's Posts Mark Forums Read

Gene Therapy Discuss gene therapy issues, methods and protocols for viral gene therapy, problems and solutions.


Sequencing reaction

Sequencing reaction - Gene Therapy

Sequencing reaction - Discuss gene therapy issues, methods and protocols for viral gene therapy, problems and solutions.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-06-2010, 11:45 PM
Pipette Filler
Points: 46, Level: 1 Points: 46, Level: 1 Points: 46, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Sequencing reaction



The image of sequencing my gene showed that there were high peaks of vector gene, but very low peaks of my inserted DNA. why ?
My gene was ligated in the PET vector. I used M13F to do the sequencing reaction.
Reply With Quote
  #2  
Old 12-07-2010, 12:23 PM
Graduate Student
Points: 2,626, Level: 33 Points: 2,626, Level: 33 Points: 2,626, Level: 33
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Location: Santiago, Chile
Posts: 100
Thanks: 1
Thanked 24 Times in 24 Posts
Default Re: Sequencing reaction

I'm sorry, but I don't understand the question... you mean you have low amounts of your vector of interest compared to religated vector?
Reply With Quote
Reply

Tags
reaction , sequencing


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
What does order of a reaction tell us? SNUMBER6 Chemistry Forum 29 06-25-2013 04:21 PM
Sequencing troubles of a PCR product fatimelopes PCR - Polymerase Chain Reaction Forum 2 09-23-2008 03:06 PM
Reaction mechanism and reaction products zhangtao@gmail.com Organic Chemistry Forum 0 12-19-2005 09:07 AM
Sequencing - Low Product Melody Protocols and Methods Forum 1 09-02-2005 11:14 AM
combustion/ionisation physics Peter Lowrie Physics Forum 3 06-20-2005 01:56 PM


All times are GMT. The time now is 03:35 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11496 seconds with 16 queries