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The image of sequencing my gene showed that there were high peaks of vector gene, but very low peaks of my inserted DNA. why ?
My gene was ligated in the PET vector. I used M13F to do the sequencing reaction.
Re: Sequencing reaction
I'm sorry, but I don't understand the question... you mean you have low amounts of your vector of interest compared to religated vector?
|reaction , sequencing|
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