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#1
12-06-2010, 10:45 PM
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 Sequencing reaction The image of sequencing my gene showed that there were high peaks of vector gene, but very low peaks of my inserted DNA. why ? My gene was ligated in the PET vector. I used M13F to do the sequencing reaction.  |
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#2
12-07-2010, 11:23 AM
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| Re: Sequencing reaction I'm sorry, but I don't understand the question... you mean you have low amounts of your vector of interest compared to religated vector? |
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