Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Regional Molecular Biology Discussion > Forum de Biologie Moleculaire
Register Search Today's Posts Mark Forums Read

Forum de Biologie Moleculaire Forum de Biologie Moleculaire. Speak French Molecular Biology Forum.


Déparaffinage

Déparaffinage - Forum de Biologie Moleculaire

Déparaffinage - Forum de Biologie Moleculaire. Speak French Molecular Biology Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-21-2007, 08:40 AM
Pipette Filler
Points: 1,060, Level: 18 Points: 1,060, Level: 18 Points: 1,060, Level: 18
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2007
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default Déparaffinage



Salut, jai des prélevements de tissus dans des cubes de paraffine, je voudrai extraire ces tissus et les conserver a -20°C pour une utilisation ulterieur (extraction d'ADN). comment dois je proceder pour le deparaffinage ?
mercii d'avance.
Reply With Quote
  #2  
Old 09-21-2007, 05:49 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: Déparaffinage

Cut 10-20 um sections. Heat at 60 degrees for 30 minutes, immerse in Hemo-De or Citrisolve, for a total of 15 minutes with 3 changes. Rehydrate by immersion in graded ethanols (100%, 95%, 75%, 50%) then water for 5 minutes each. Then immerse in PBS for 5 minutes. Ready for Proteinase K.

La coupure 10-20 sections d'um. Chauffer * 60 degrés pour 30 minutes, immerger dans Hemo-De ou Citrisolve, pour un total de 15 minutes avec 3 changements. Rehydrate par l'immersion dans les éthanols gradués (100%, 95%, 75%, 50%) alors l'eau pour 5 minutes chaque. Alors immerger dans PBS pour 5 minutes. Prêt pour Proteinase K.
Reply With Quote
  #3  
Old 04-01-2008, 09:30 AM
Pipette Filler
Points: 1,060, Level: 18 Points: 1,060, Level: 18 Points: 1,060, Level: 18
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2007
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Déparaffinage

Thankss !!!!
Reply With Quote
  #4  
Old 04-01-2008, 09:32 AM
Pipette Filler
Points: 1,060, Level: 18 Points: 1,060, Level: 18 Points: 1,060, Level: 18
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2007
Posts: 6
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Déparaffinage

thanks !!
Reply With Quote
Reply

Tags
déparaffinage


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Déparaffinage !! hait77 Basic Lab Protocols and Techniques 2 09-05-2007 08:11 AM


All times are GMT. The time now is 08:30 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12637 seconds with 16 queries