Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Forum Chemie (http://www.molecularstation.com/forum/forum-chemie/)
-   -   Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification (http://www.molecularstation.com/forum/forum-chemie/9567-determination-oxytocin-biological-samples-isocratichigh-performance-liquid-chromatography-coulometric-detection-using-c18solid-phase-extraction-polyclonal-antibody-based-immunoaffinity-columnpurification.html)

tocopherol 03-21-2008 07:40 PM

Determination of oxytocin in biological samples by isocratichigh-performance liquid chromatography with coulometric detection using C18solid-phase extraction and polyclonal antibody-based immunoaffinity columnpurification
 
Determination of oxytocin in biological samples by isocratic high-
performance liquid chromatography with coulometric detection using C18
solid-phase extraction and polyclonal antibody-based immunoaffinity
column purification. J Chromatogr B Biomed Appl. 1994 Mar 4;653(2):
139-45.

Kukucka Mark A. and Misra, Hara P.

Department of Biomedical Sciences, Virginia-Maryland Regional College
of Veterinary Medicine, Virginia Polytechnic Institute and State
University, Blacksburg 24061-0442.

ABSTARCT

A specific high-performance liquid chromatographic (HPLC) method is
described for the reliable quantitation of oxytocin using culture
media supernatants. The procedure employs solid-phase extraction,
antibody-based immunoaffinity purification and isocratic HPLC with
dual channel coulometric detection (ED). The lower limit of detection
for this cyclic nonapeptide was 40 pg (40 fmol). Due to its relative
simplicity, specificity and precision, the HPLC-ED of oxytocin is an
accurate and attractive alternative to many existing quantitative
methods.

felipe 10-10-2012 02:11 PM

Re: Determination of oxytocin in biological samples by isocratichigh-performance liqu
 
Are there really various methods to detect oxytocin? Can you please explain these methods briefly? I would like to know about them, actually i am interested to know this.


All times are GMT. The time now is 07:38 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08958 seconds with 11 queries