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procedure/protocolli per prelievi di sangue

procedure/protocolli per prelievi di sangue - Forum Biologia

procedure/protocolli per prelievi di sangue - Forum Scienza Biologia. Italian Biology Forum.


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Old 01-18-2006, 06:53 PM
tex
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Default procedure/protocolli per prelievi di sangue



buona sera, chiedo se qualcuno è a conoscenza di protocolli o procedure
nazionali per i prelievi di sangue nei laboratori di analisi cliniche.
ringrazio chi vorrà rispondere.
tex




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Old 01-18-2006, 09:57 PM
Morgan
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Default procedure/protocolli per prelievi di sangue


"tex" <[Only registered users see links. ]> ha scritto nel messaggio
news:dqm2qu$p64$[Only registered users see links. ]...
Dipende dal tipo di prelievo e cmq non sono
codificati per legge ,se è questo che intendi ,a livello nazionale.
Esistono delle linee guida per la raccolta di determinati
campioni biologici ,penso per esempio alla Bence Jones di
cui puoi trovare le linee guida sul sito della SIBIOC e delle procedure
che puoi trovare su qualsiasi manuale di laboratorio.
Poi c'è tutto il discorso della qualità e certificazioni varie
ma stendiamo un velo pietoso.
Come mai chiedi questo?

Morgan


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Default Re: procedure/protocolli per prelievi di sangue

Bence Jones TSA screen test method
TSA precipitates Bence-Jones protein even in such small amounts as 0.3 mg/dl of urine. It does not precipitate albumin in much higher concentrations than 25 g/dl; it precipitates globulins in concentrations higher than 5 mg/dl of urine. The reagent should be added slowly by allowing it to run down the side of the test tube. The tube is then "finger-flicked." If a precipitate occurs within 5 minutes, the test is positive for Bence-Jones protein.
Procedure
Centrifuge 10 ml fresh urine at 1000 g for 10 min. Dispense 1 ml supernatant in short tube (100x11 mm), and slowly layered on top of 0.5 ml precipitating solution ( 12 g p-toluene sulfonic acid [TSA] in 100 ml glacial acetic acid). Volumes of the components can be proportionally varied without result modified. In some cases, thick creamy or turbid precipitate is seen on the interface, the test tube is put in boiling water. The precipitate completely disappeared after a few minutes only in case with BJP and reappeared at room temperature. All positive screening tests should be confirmed by performing serum protein electrophoresis. The sample is normally concentrated prior to protein gel electrophoresis and the test supplemented with immunofixation techniques
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