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como preparo cresyl violet

como preparo cresyl violet - Foro Biologia Molecular

como preparo cresyl violet - Foro Biologia Molecular. Speak Spanish Molecular Biology Research Forum.


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Old 02-15-2007, 07:00 PM
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Default como preparo cresyl violet



Quien pueda decirme como preparar el reactivo Cresyl violet que se usa en tincion de tejidos ... mil gracias
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Old 07-04-2007, 04:29 PM
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Default Re: como preparo cresyl violet

Mira, hace un tiempo atrás usé un protocolo para tinción de cuerpos de Nissl sacado de un reactivo de merck.

Esta era disolviendo 0.1g de cresil violeta en 75 ml de agua destilada. despues dilu*amos 6 ml de esta solucion en 50 mL de buffer acetato 0.1 M pH 3.6
(Solucion A (Acetato de sodio 0.1 M)= 8.2 g de acetato de Na anhidro en 1 L de agua destilada / solucion B (Acido acético 0.1 M)= 5.8 g de acido acético glacial en 1 L de agua destilada).

Espero que te sea util.

Nos vemos
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