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mismos primers distinto producto en PCR y qPCR??

mismos primers distinto producto en PCR y qPCR?? - Foro Biologia Molecular

mismos primers distinto producto en PCR y qPCR?? - Foro Biologia Molecular. Speak Spanish Molecular Biology Research Forum.


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Old 08-14-2012, 08:35 PM
Pipette Filler
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Default mismos primers distinto producto en PCR y qPCR??



Hola!

Bueno, lo he puesto también en el foro de inglés pero por si acaso...

He hecho una qPCR para probar unos primers nuevos y he obtenido amplificación y curvas de melting como las esperaba. Además cargué un gel de agarosa para ver el tamaño del producto y también me ha salido como esperaba (180 bp)

El problema ha sido que al hacer una PCR convencional para mandarlo a secuenciar, he cargado otro gel para comprobar el producto y me ha salido uno enorme (1000 bp).

¿Alguien sabe por qué usando los mismos primers y mismas muestras en la qPCR sale bien y en la convencional obtengo un producto que no es?

Mil gracias!
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Old 09-18-2012, 03:05 AM
Pipette Filler
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Default Re: mismos primers distinto producto en PCR y qPCR??

I can't get what you've said's meaning. Can you speak English, thanks!
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distinto , mismos , pcr , primers , producto , qpcr


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