Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Regional Molecular Biology Discussion > Foro Biologia Molecular
Register Search Today's Posts Mark Forums Read

Foro Biologia Molecular Foro Biologia Molecular. Speak Spanish Molecular Biology Research Forum.


calculos

calculos - Foro Biologia Molecular

calculos - Foro Biologia Molecular. Speak Spanish Molecular Biology Research Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-16-2009, 08:30 PM
Pipette Filler
Points: 155, Level: 3 Points: 155, Level: 3 Points: 155, Level: 3
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2009
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default calculos



Hola soy nueva en este foro alguien pude proporcionarme alguna tabla de conversiones, para calculos moleculares. Es que tengo que correr una pcr y es la primera vez que lo hago y estoy batallando con los calculos para prepara los primes y la taq.

Se los agradecere mucho.

Atte

lupita (soy mexicana)
Reply With Quote
  #2  
Old 08-18-2009, 08:12 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: calculos

ejemplo para preparar solucion concentrada the primers:
A) GAPDH Fwd; 78.50nmoles (primer liofilizado)
V=78.50nmoles/0.5nmol/ul =157ul H2O
*hace 157ul de primer a concetracion de 0.5nmol/ul (que es 500uM)

B)GAPDH Rev; 77.90nmoles (primer liofilizado)
V=77.90nmoles/0.5nmoles/ul =155.8ul H2O
*hace 155.8ul de primer a concetracion de 0.5nmol/ul (que es 500uM)

Ejemplo para hacer solucion diluida the primers:
*Haz una dilucion de 1:10 de primers concentrados
**la concentracion va de 500uM(primer concentrado) a 50uM (primer diluido)

Ejemplo para hacer PCR mix:

3. PCR Mix
Reagent-------- [Initial]-- [Final] Volume
10X PCR buffer- 10X----- 1X--- 2.5Ál
10X dNTPs----- 2mM---- 0.2mM--- 2.5Ál
Primer fwd----- 50ÁM--- 0.5ÁM--- 1Ál
Primer rev----- 50ÁM--- 0.5ÁM--- 1Ál
Taq---------- 5U/Ál--- 1.25U--- 0.25Ál
H2O--------- -------- -------- 15.25Ál
DNA--------- -------- -------- 2.5Ál
-------------------------------------25Ál
PCR Thermocycle Program
a. 95░ 5min
b. 94░ 45sec
c. 55░ 45sec
d. 72░ 45sec
e. back to b 35 times
f. 72░ 5min
g. 4░ hold

Last edited by danfive; 08-18-2009 at 09:23 PM.
Reply With Quote
The Following User Says Thank You to danfive For This Useful Post:
admin (05-15-2010)
  #3  
Old 05-14-2010, 05:40 PM
Pipette Filler
Points: 30, Level: 1 Points: 30, Level: 1 Points: 30, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2010
Posts: 5
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: calculos

sabes convertir cc en ppb?
Reply With Quote
Reply

Tags
calculos


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 04:23 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12164 seconds with 15 queries