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bacterial cells not surviving flow sorting

bacterial cells not surviving flow sorting - Flow Cytometry Forum

bacterial cells not surviving flow sorting - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 04-18-2013, 08:20 PM
Pipette Filler
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Default bacterial cells not surviving flow sorting



Hello,

I am sorting E. coli cells by FACS. I sort my target population into recovery media. Sorted cells are then spread on agar plates and grown overnight. I am getting a very low percentage of colonies on the agar plates, about 2-10% of the colony numbers that I expect. Has anyone else experienced this? Anyone have any suggestions as to why this may be happening and what to do about it? thanks.
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bacterial , cells , e. coli , facsaria , flow , sorting , survival , surviving


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