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% positivity - gating or overton?

% positivity - gating or overton? - Flow Cytometry Forum

% positivity - gating or overton? - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


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Old 03-30-2012, 12:13 PM
Pipette Filler
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Question % positivity - gating or overton?



Hi there,
I am trying to gate a population of cells that display low-level positivity. While the overton subtraction method may be more sensitive, I wonder is it correct to use simple marker/gating also?
Thanks a mill!
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