Using the FCAS; I ran some stained cells. The same cell types were stained with directly labelled antibody and corresponding isotype controls (as recommended by the company).
however some of the isotype controls produced slightly higher signals than the corresponding samples.
Why is this ... just small differences in binding between isotype and antibody?
Which (isotype or antibody signal) should I assume to be correct if I am
using this sample as my untreated control?
Any info at all would be helpful!! Many thanks