we have three peaks when doing cell cycle analysis using propidium iodide.
We are looking at U87 glioblastoma cell lines
(ATCC: HTB-14), for which the ATCC info sheet states: "This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%."
The mean channel number of the second peak is about 2x of the first one and the third 2x of the second. So it looks like cells with 2n / 4n chromosome number, right? How can we then look for apoptosis by DNA fragmentation (sub-G1)?
Any help is greatly appreciated!