|Register||Search||Today's Posts||Mark Forums Read|
|Flow Cytometry Forum Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.|
| ||LinkBack||Thread Tools||Display Modes|
What is the shear forces on beads (or cells) passing through a FACS instrument
Is there any way to determine this from first principles of field theory? I suspect a pretty rigorous understanding of FACS sorting is required, but although I understand the theory I don't even know where to start to determine the forces.
Are the forces enough to lyse cells? What is the minimum affinity of a ligand to its receptor on a bead that will stay attached through the process?
Re: What is the shear forces on beads (or cells) passing through a FACS instrument
FACS machines suspend the cells in sheath fluid; the main purpose of this fluid is to reduce/eliminate shear forces on the cells. As such, the shear experienced by cells tends to be quite low, although the exact value will vary from model-to-model. Because of the sheath fluid there is no easy way to calculate the shear forces involved.
That said, the forces are small enough that most FACS machines will not disrupt weak interactions between particles and cells; we often measure cell-cell binding for phagocytosis assays using FACS, and extracellular cells, often held in place by a small number of modest affinity receptors, remain attached.
If you need to know the absolute shear values involved, you're best off contacting th technical support of your FACS machine's manufacturer. They should have this information.
|beads , cells , facs , forces , instrument , passing , shear , shear forces|
|Thread||Thread Starter||Forum||Replies||Last Post|
|mouse hepatocytes isolation||amicia||Cell Biology and Cell Culture||39||05-11-2012 11:52 AM|
|Characteristics of cancerous cells||aftabac||Cell Biology and Cell Culture||2||08-26-2011 11:40 PM|
|Human Cytome Project - Update 24 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||1||08-01-2010 02:18 PM|
|Production of Monoclonal Antibodies - Technique in Mouse||Cellbiogal||Antibody Forum||2||01-08-2009 09:52 PM|
|Human Cytome Project - Update 6 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||0||01-06-2005 10:18 AM|