Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Flow Cytometry Forum
Register Search Today's Posts Mark Forums Read

Flow Cytometry Forum Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


FACS sorting Langerhans cells out of the skin

FACS sorting Langerhans cells out of the skin - Flow Cytometry Forum

FACS sorting Langerhans cells out of the skin - Flow Cytometry Forum. Discuss FACS cell sorting, flow cytometry, analysis and cell staining.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-03-2011, 08:25 AM
Pipette Filler
Points: 24, Level: 1 Points: 24, Level: 1 Points: 24, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default FACS sorting Langerhans cells out of the skin



Hi mates...

I am experiencing troubles if we try to isolate Langerhans cells out of murine epidermal cell suspension...we tried to FACS sort them with an FACS ARIA I..
Does anyone of you already tried sorting Langerhans cells out of the skin? We´ve already tried different approaches regarding EC suspension (adding EDTA or DNase I for preventing clumps...) as welll as different settings for the FACS..however we are only reaching about 25 to 30% purity...anyone detailed instrument settings or any idea..?

thx and cheers

playalistica

Last edited by Playalistica; 03-03-2011 at 08:34 AM.
Reply With Quote
Reply

Tags
cells , facs , langerhans , skin , sorting


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Characteristics of cancerous cells aftabac Cell Biology and Cell Culture 2 08-26-2011 11:40 PM
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
How to isolate adult from circulation? peterish Stem Cell Forum 0 05-19-2008 10:02 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 03:54 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14382 seconds with 16 queries